Assistant Professor (on contract)
+919947797405
mohindm5@gmail.com
Qualifications : M.Sc., Ph.D., NET
I worked as Junior research fellow and as Senior research fellow in a DST and DBT, Government of India, funded project in Identification of anti-inflammatory bioactive molecules from Ayurvedic medicines. The project involved the identification of the natural molecules and in delineating the mechanism of action in controlling inflammatory pathways. The compounds were isolated by means of chromatographic techniques (HPLC and HPTLC) and were identified by means of high-resolution mass spectrometry (LCMS qTof). The in silico Pharmacokinetic properties of the identified compounds were carried out using ADMET tools. The interaction between the identified compounds and various protein targets and enzymes involved in the inflammatory pathways was determined by in silico molecular docking. In order to identify the mode of action of these compounds in controlling inflammatory pathways, high throughput in vitro enzyme inhibition assays and animal cell culture models were employed. Gene expression studies of various inflammatory genes were done and also the effects of compounds on proinflammatory cytokine pathway were found out.I was actively involved in understanding/ identifying the pathways and bioactive constituents responsible for reducing obesity by an Ayurvedic herbal formulation. The study consists of both in vivo (male Spraque Dawley rats) and in vitro cell culture (3t3l cell lines) experiments. Studies were done on the conversion of preadipocytes to adipocytes, intracellular lipid accumulation by various molecular and fluorescent techniques. The gene expression of various adipokines and other genes was examined. Glucose uptake studies (fluorescently tagged glucose uptake) and proteomics (by LCMS) were also done. The lipid-lowering effect in high-fat diet-fed male SD rats was also carried out. The study also involved the interaction of immune cells with adipose tissues.Besides the above, I have collaborated in determining the anti-inflammatory activity of two plants Elephantopus scaber and Tinospora cordifolia..